一、基本信息
李壮，博士
电子邮件：zhuangli@hubu.edu.cn
研究领域：生物化学、结构生物学。运用单颗粒冷冻电子显微镜技术，辅助其它生物物理和生物化学的方法，研究CRISPR-Cas系统相关复合物的结构基础和功能机制。
二、教育背景
2012.09– 2018.01 北京大学，博士
2008.09–2012.07 西北大学，本科
三、工作经历
2021.10至今 3344体育会员
2021.03– 2021.9 哥伦比亚大学，博士后
2018.30–2021.03 普渡大学，博士后
四、代表性论文（#共同第一作者）
1. Xiao R#, Wang S#, Han R, Li Z, Gabel C, Mukherjee I, Chang L. (2021) Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition. Molecular Cell doi.org/10.1016/j.molcel.2021.07.043
2. Chen Q, Plasencia M, Li Z, Mukherjee S, Patra D, Chen C, Klose T, Yao X, Kossiakoff A, Chang L, Andrews P, Tesmer J. (2021) Structure of a G protein-coupled receptor kinase engaging its GPCR. Nature 595, 600–605.
3. Li Z#, Zhang H#, Xiao R#, Han R, Chang L. (2021) Cryo-EM structure of the RNA-guided ribonuclease Cas12g. Nature Chemical Biology 17, 387–393.
4. Xiao R#, Li Z#, Wang S, Han R, Chang L. (2021)Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR-Cas12f nuclease. Nucleic Acids Res 49, 4120–4128.
5. Guntupalli SR#, Li Z#, Chang L, Plapp BV, Subramanian R. (2021) Cryo-electron microscopy structures of yeast alcohol dehydrogenase. Biochemistry 60, 663–677.
6. Gabel C#, Li Z#, Zhang H, Chang L. (2020) Structural basis for inhibition of the type I-F CRISPR-Cas surveillance complex by AcrIF4, AcrIF7, and AcrIF14. Nucleic Acids Research 49, 584–594.
7. Zhang H#, Li Z#, Xiao R#, Chang L. (2020) Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease. Nature Structural & Molecular Biology 27, 1069–1076.
8. Li Z#, Zhang H#, Xiao R, Chang L. (2020) Cryo-EM structure of a type I-F CRISPR RNA guided surveillance complex bound to transposition protein TniQ. Cell Research 30, 179-181.
9. Zhang H#, Li Z#, Daczkowski CM, Gabel C, Mesecar AD, Chang L. (2019) Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins. Cell Host Microbe 25, 815-826.
10. Li Z, Zhao D, Xiang B, Li H. (2017) Structural and biochemical characterization of DAXX-ATRX interaction. Protein Cell 8, 762-766.
五、招生招聘
热忱欢迎对本实验室研究方向感兴趣的本科生前来攻读博士学位或者进行本科毕业论文研究。
