张丽兰、博士

主要研究方向：酶结构生物学

电子邮件：zhang_lilan@163.com

通讯地址：湖北省武汉市武昌区友谊大道368号3344体育会员

一、研究领域：结构生物学、酶结构及机理研究、酶定向改造

使用结构生物学手段对具有重要功能的蛋白质进行结构和机理研究，并基于结构进行理性设计和定向改造，主要包括：（1）萜类合成酶；（2）细胞色素P450酶；（3）工业酶。目前已在Nature Communications、Angew. Chem. Intl. Ed、JACS和ACS Catalysis等SCI期刊发表论文14篇，其中3篇获选为封面文章。申请专利1项，2019年获天津市自然科学二等奖1项。

二、教育背景

（1）2016.09-2019.07，中国科学院大学（中国科学院天津工业生物技术研究所），生物化学与分子生物学专业，博士，导师：郭瑞庭教授；

（2）2012.03-2014.03，中国科学院大学（中国科学院天津工业生物技术研究所），联合培养，硕士，导师：郭瑞庭教授；

（3）2011.09-2012.02，天津科技大学，轻工技术与工程（发酵）专业，硕士，导师：张娟琨教授；

（4）2007.09-2011.07，兰州理工大学，生物工程专业，本科，导师：张新国教授。

三、科研与学术工作经历

（1）2019.07-至今，湖北大学、3344体育会员，博士后，合作导师：郭瑞庭教授；

（2）2014.04-2016.08，中国科学院天津工业生物技术研究所，助理研究员。

四、发表文章和专利

1.Chen, C. C., Han, X., Li, X., Jiang, P., Niu, D., Ma, L., Liu, W., Li, Siyu, Qu, Y., Hu, H., Min, J., Yang, Y.,Zhang, L.,Zeng, W., Huang, J. W.,* Dai, L.,*Guo, R. T.*General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis,Nature Catalysis,2021,4, 425-430(IF 30.471, Highlighted by ChemistryViews,科技日报,中国科学报)

2.Chen, C. C., Min, J.,Zhang, L.,Yang, Y., Yu, X.,*Guo, R. T.*Advanced Understanding of the Electron Transfer Pathway of Cytochrome P450s.ChemBioChem,2021,22, 1317-1328(Review,Coverstory)

3.Zhang, L.,^#Xie, Z.,^#Liu, Z.,^#Zhou, S., Ma, L., Liu, W., Huang, J. W., Ko, T. P., Li, X., Hu, Y., Min, J., Yu, X.,Guo, R. T.,*Chen, C. C.* Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.Nature Communications,2020,11, 2676.(IF 12.12, Highlighted by C&EN, ChemistryViews,科技日报,中国科学报)

4.Chen, C. C.,*Zhang, L.,Yu, X., Ma, L., Ko, T. P.,Guo, R. T.*Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation.ACS Catalysis,2020,10, 4717-4725.(IF 12.350, Review, ACS Editors’ Choice, Cover story)

5.Chen C. C.,^#Xue J.,^#Peng W.,^#Wang, B.,Zhang L.,Liu W., Ko T.-P., Huang, J. W., Zhou S., Min J., Ma L., Dai L., * Guo R. T.* Structural insights into thebaine synthase 2 catalysis.Biochemical and Biophysical Research Communications,2020,529 (2), 156-161.

6.Huang, J. W., Niu, D., Liu, K., Wang, Q., Ma, L., Chen, C. C.,Zhang, L.,Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R. T.*Structure basis of non-structural protein pA151R from African Swine Fever Virus.Biochemical and Biophysical Research Communications,2020,532, 108-113.

7.Zhang, L.,^#Ko, T. P.,^#Malwal S. R.,^#Liu W., Zhou S., Oldfield E.,Guo R. T.,*and Chen, C. C.*Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism,Biochemical and Biophysical Research Communications,2019,511, 800-805.

8.Yang Z.,^#Zhang L.,^#Yu X., Wu S., Yang Y., Hu Y., Li Q., Shang N., Guo R. T., Chen C. C., Dai L.,* Liu W.* Crystal structure of TchmY from Actinoplanes teichomyceticus.Acta Crystallographica Section F: Structural Biology Communications,2019,75(9): 570-575.(共同第一作者)

9.Ma, J.,^#Ko, T. P.,^#Yu, X.,^#Zhang, L.,Ma, L., Zhai, C.,Guo, R. T.,Liu, W., Li, H., Chen, C. C.* Structural insights to heterodimericcis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1.Biochemical and Biophysical Research Communications,2019,515,621-626.

10.Chang, Z.,^#Ansbacher, T.,^#Zhang, L.,^#Yang, Y., Ko, T. P., Zhang, G., Liu, W., Huang, J. W., Dai, L.,Guo, R. T.,Major, D. T.,* Chen, C. C.* Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis,Organic Biomolecular Chemistry,2019,17, 2070-2076.(Cover story,共同第一作者)

11.Zhang, L.,^#Chen, C. C.,^#Ko, T. P.,^#Huang, J. W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, Ke., Huang, C. C., Hsu, S. T., Wang, A. H., Oldfield, E.,* andGuo, R. T.*Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5,Angewandte Chemie International Edition,2016,55,4716-4720.(IF 12.257)

12.Chan H. C.,^#Feng X.,^#Ko T. P.,^#Huang C. H., Hu Y., Zheng Y., Bogue S., Nakano C., Hoshino T.,Zhang L.,Lv P., Liu W., Dean C. Crick, Liang P. H., Wang, A H., Oldfield E.,* Guo R. T.* Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase fromMycobacterium tuberculosis.Journal of the American Chemical Society,2014,136(7): 2892-2896.(IF 14.612)

13.Lv P.,^#Zhang L.,^#Luo H.,^#Chen C. C., Huang C.-H., Peng W., Wang K., Ko T.-P., Zheng Y., Zhang J., Bin Yao.,*Guo R. T.* Preliminary X-ray diffraction analysis of thermostable β-1, 4-xylanase fromStreptomyces sp. S9.Acta Crystallographica Section F: Structural Biology Communications,2014,70(1): 105-107.(共同第一作者)

14.Zhang L.,^#Zhao P.,^#Chen C. C.,^#Huang C. H., Ko T.P., Zheng Y., Guo R. T.* Preliminary X-ray diffraction analysis of a thermophilic β-1, 3–1, 4-glucanase fromClostridium thermocellum.Acta Crystallographica Section F: Structural Biology Communications,2014,70(7): 946-948.

15.Chen, C. C., Huang, J. W., Dai, L. H., Yu, X. J., Chen, C. Y., Wu, S., Zhan, Z. C.,Zhang, L. L.,Zhai, C., Ma, L. X., Guo, R. T.Cellulase having improved enzymatic activity，授权号：US10479985B1，美国专利。

代表性封面文章

五、奖励与荣誉称号

2021年“湖北省楚天学子”

2020年“湖北省创新群体”

2019年“天津市自然科学二等奖”

六、主持或参加科研项目情况

1.国家重大专项（青年项目）、2021/01-2023/12，60万、在研、项目骨干；

2.自然科学基金（青年基金）项目、2021/01-2023/12，24万、在研、主持；

3.中国博士后特别资助（站中）项目、2020.07-2021.08，18万、在研、主持；

4.中国博士后面上项目、2019.10-2021.08，8万、在研、主持；

5.湖北省科技活动项目择优资助、2019.10-2021.08，2万、在研、主持；

6.国家重大专项、2019/12-2024/11，552万、在研、参与

7.湖北省创新群体、2020/3-2023/3，50万、在研、参与