一、基本信息
吴姗，博士，教授，博士研究生导师
电子邮件：wushan91(AT)hubu.edu.cn
研究领域：单颗粒冷冻电镜三维重构技术，生化与分子生物学，结构生物学。利用冷冻电镜三维重构技术解析生物体内重要酶以及大型复合物的精细结构，结合遗传学、生化与分子生物学、细胞生物学等手段阐明分子机制。
二、教育背景
2013–2018 清华大学，生物学，博士
2009–2013 华中师范大学，化学生物学交叉培养班，本科
三、工作经历
2019至今 3344体育会员，教授
四、科研项目
1. 国家自然科学基金（31900930）：真核核糖体组装因子Sda1的结构和功能研究，2020.01–2022.12，主持
2. 重点研发计划（蛋白质机器与生命过程调控）2016YFA0500703：染色质结构的动态变化及调控机制研究，2016.07–2020.12，参与
3. 国家自然科学基金（31470722）：真核核糖体组装过程的结构和功能研究， 2015.01–2018.12，参与
五、代表性论文（#共同一作，*通讯作者）
1. Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S*, Zou T*. (2022) Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism. Nature Microbiology doi.org/10.1038/s41564-022-01197-7
2. Su N, Zhu A, Tao X, Ding ZJ, Chang S, Ye F, Zhang Y, Zhao C, Chen Q, Wang J, Zhou CY, Guo Y, Jiao S, Zhang S, Wen H, Ma L, Ye S, Zheng SJ, Yang F*, Wu S*, Guo J*. (2022) Structures and mechanisms of the Arabidopsis auxin transporter PIN3. Nature doi.org/10.1038/s41586-022-05142-w
3. Wu S, Tutuncuoglu B, Yan Kaige, Brown H, Zhang Y, Tan D, Gamalinda M, Yuan Y, Li Z Jakovljevic J, Ma C, Lei J, Dong M, Woolford JL Jr, Gao N. (2016). Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes. Nature 534, 133–137. (SCI, IF=40.137)
4. Wu S, Tan Dan, Woolford JL Jr, Dong M, Gao N. (2017). Atomic modeling of the ITS2 ribosome assembly subcomplex from cryo-EM together with mass spectrometry-identified protein-protein crosslinks. Protein Science 26, 103–112. (SCI, IF=2.523)
5. Wang J#, Wang J#, Hu M#, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang H*, Zhou J*, Chai J*. (2019). Ligand–triggered allosteric ADP release primes a plant NLR complex. Science 364, 1–12.(SCI, IF=41.058)
6. Yang Z#, Zhang Lilan#, Yu X, Wu S, Yang Y, Hu Y, Li Q, Shang N, Guo RT, Chen CC, Dai L*, Liu W*. (2019) Crystal structure of TchmY from Actinoplanes teichomyceticus. Acta Crystallographica Section F Structural Biology Communications 75, 570–575.
7. Yuan Y#, Du C#, Sun C#, Zhu J, Wu S, Zhang Y, Ji T, Lei J, Yang Y, Gao Ning*, Nie G*. (2018). Chaperonin-groel as a smart hydrophobic drug delivery and tumor targeting molecular machine for tumor therapy. Nano Letters 18, 921–928. (SCI, IF=12.08)
8. Ma C, Wu S, Li N, Chen Yan, Yan K, Li Z, Zheng L, Lei J, Woolford JL Jr*, Gao Ning*. (2017) Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nature Structural & Molecular Biology 24, 214–220. (SCI, IF=12.595)
9. Biedka S, Wu S, LaPeruta AJ, Gao N, Woolford JL Jr. (2017). Insights into remodeling events during eukaryotic large ribosomal subunit assembly provided by high resolution cryo-EM structures. RNA Biology 14, 1306–1313. (SCI, IF=3.9)
10. Tutuncuoglu B, Jakovljevic J, Wu S, Gao N, Woolford JL Jr. (2016). The N-terminal extension of yeast ribosomal protein L8 is involved in two major remodeling events during late nuclear stages of 60S ribosomal subunit assembly. RNA 22, 1386–1399. (SCI, IF=4.605)
11. Zhang D, Yan K, Liu G, Song G, Luo J, Shi, Y, Cheng E, Wu S, Jiang T, Lou J, Gao N, Qin Y. (2016). Ef4 disengages the peptidyl-trna cca end and facilitates back-translocation on the 70s ribosome. Nature Structural & Molecular Biology 23, 125–131. (SCI, IF=12.595)
12. Sun C#, Yuan Y#, Xu Z, Ji T, Tian Y, Wu S, Lei J, Gao N*, Nie G*. (2015). Fine-tuned H-ferritin nanocage with multiple gold clusters as near-infrared kidney specific targeting nanoprobe. Bioconjugate Chemistry 26, 193–196.
13. Zhang Y, Ma C, Yuan Y, Zhu J, Li N, Chen C, Wu S, Yu L, Lei J, Gao N. (2014). Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome. Nature Structural & Molecular Biology 21, 1042–1046. (SCI, IF=12.595)
14. Chen CC, Huang JW, Dai LH, Yu XJ, Chen CY, Wu S, Zhan ZC, Zhang L L, Zhai C, Ma LX, Guo RT. Cellulase having improved enzymatic activity，授权号：US10479985B1，美国专利 。
六、招贤纳士
本课题组招收对结构生物学以及本课题组研究方向感兴趣的硕士以及博士研究生。常年欢迎对科学感兴趣的本科生进入实验室学习。
